Automated PFAS Sample Preparation for EPA 537.1, 533, 1633A & DIN EN 17892

Per- and Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by E. Walters
Biotage, 2210 Ramsey Circle, Schaumburg, IL, 60194, United States


Contact Information: [email protected]; 603-401-6266

ABSTRACT

PFAS analysis requires highly controlled, contamination-free sample preparation across a wide range of matrices. This work introduces an automated solid phase extraction (SPE) approach designed to improve robustness and versatility in PFAS workflows. An initial comparison of three automated SPE platforms highlights key differences in configuration, throughput, and suitability for common PFAS applications. The technical focus examines automated SPE performance for established PFAS methods, including DIN EN 17892, EPA 1633A, EPA 533, and EPA 537.1. Method performance characteristics such as detection limits, precision, and accuracy are discussed across representative matrices. The role of advanced sorbent chemistries, including ISOLUTE PFAS+ and EVOLUTE WAX/GCB, is evaluated with respect to background levels, recoveries, and overall method reliability. Operational considerations relevant to routine PFAS laboratories are also addressed. The impact of automation on laboratory throughput, analyst handling, and contamination control is assessed, including strategies to minimize carryover through integrated cleaning procedures. Emphasis is placed on practical implementation and method robustness rather than system-specific features. Overall, this work provides technical insight into automated SPE strategies for PFAS sample preparation and demonstrates how the Biotage PrepXpert-8 fits within a broader automated SPE portfolio to support regulatory compliance, analytical performance, and laboratory efficiency.