Trace aldehydes in alcohol and drinking water using on-fiber derivatization SPME Arrow and GC/TQDrinking Water
Poster Presentation
Prepared by A. Cuthbertson, E. O'Neill, M. Curtis
Agilent, 2850 Centerville Rd, Wilmington, DE, 19805, United States
Contact Information: [email protected]; 302-332-6928
ABSTRACT
Aldehydes are influential markers of oxidation and aroma in fermented beverages and are also encountered at trace levels in drinking water, where they can arise from raw water precursors and treatment or storage processes. Their volatility, polarity, and chemical reactivity complicate trace level quantification, especially across carbonyls of differing chain length. We present a sensitive, low touch workflow that couples on fiber derivatization with O (2,3,4,5,6 pentafluorobenzyl)hydroxylamine (PFBHA) to SPME Arrow extraction and GC–triple quadrupole (Agilent 8890/7000E) operated in dynamic MRM (dMRM). PFBHA rapidly forms stable oximes that are highly amenable to GC–MS/MS, improving detectability and selectivity for carbonyls. The larger sorptive phase of SPME Arrow increases uptake capacity and robustness versus conventional SPME fibers, supporting higher sensitivity and throughput. Using dMRM on the 7000E enhances selectivity and minimizes interferences in complex beverage and water matrices. Sub ppb linear calibrations were achieved for C2–C9 aldehydes (acetaldehyde through nonanal). Proof of concept measurements were performed in commercial wine and drinking water with minimal sample volume and preparation.
