Automated Workflow for High-throughput PFAS Sample Preparation for Solid Matrices Following EPA Method 1633.Per- and Polyfluoroalkyl Substances (PFAS) in the Environment
Oral Presentation
Prepared by E. Walters
Biotage, 10430 Harris Oak Blvd Ste C, Charlotte, North Carolina, 28269, United States
Contact Information: [email protected]; 603-401-6266
ABSTRACT
Per- and Polyfluoroalkyl Substances (PFAS) are toxic compounds known for their persistence, potential health risks, and their ability to accumulate in living organisms, including humans. As the concern about PFAS contamination grows, analytical methods become paramount in accurately detecting and quantifying these substances across different sample types. This work focuses on an automated workflow for the extraction of PFAS in solid samples. The study evaluates the extraction performance using United States Environmental Protection Agency (USEPA) Method 1633 criteria for method detection limits (MDLs), precision, and accuracy for the determination of 40 target PFAS analytes. Sample homogenization, liquid extraction, solid phase extraction, solvent evaporation and concentration is performed in an efficient workflow utilizing the Biotage Lysera, TurboVap LV, and Extrahera HV-5000. Results demonstrate measured detection limits, precision, and accuracy exceeding EPA method 1633 acceptance criteria for initial demonstration of capability. In addition, this work outlines a simple sample preparation technique that enables high throughput processing of samples while maintaining exceptional data quality. Advantages of this workflow include elimination of manual transfer steps, complete preparation of up to 24 samples in less than four hours, and minimal cleaning required between sample batches. In conclusion, this automated workflow offers a highly efficient and reliable solution for PFAS analysis in non-drinking water samples, addressing key challenges and surpassing EPA Method 1633 performance criteria.
