Novel PFAS Analysis Using High Resolution Accurate Mass Spectrometry

Emerging Environmental Applications for High Resolution Mass Spectrometry
Oral Presentation

Prepared by C. Butt1, H. Lee2
1 - SCIEX, 500 Old Connecticut Path, Framingham, Massachusetts, 01701, United States
2 - SCIEX, 71 Four Valley Dr, Concord, Ontario, L4K 4V8, Canada

Contact Information: [email protected]; 774-375-0524


Poly- and perfluorinated alkyl substances (PFAS) have been manufactured since the 1950s but were only recognized as global environmental pollutants in 2001. Comprehensive and sensitive analytical techniques are essential to adequately determine environmental risk and quantify human exposure. This presentation explores several applications of high resolution accurate mass spectrometry to interrogate PFAS in the environment. One specific application discussed will be the use of electron activated dissociation (EAD) fragmentation for improved characterization of AFFF-derived PFAS compounds. EAD is a softer form of fragmentation as compared to traditional collision-induced dissociation (CID) and often results in the formation of diagnostic fragments that are not produced in CID. Another application that will be discussed is the use of non-targeted acquisition for the analysis of PFAS in consumer products and food. NTA techniques with large screening lists have the potential to improve compound identification through the use of MS/MS library matching, or use of MS/MS diagnostic fragments. Finally, the benefits of using MRM-HR, with monitoring high resolution fragments, to increase specificity and reduce matrix background noise will be discussed.