Comparing Extraction Protocols, Analytical Requirements and Results from Methods Utilized to Test for PFAS in Aqueous Samples: EPA 537.1, EPA 533, ISO 21675

Polyfluoroalkyl Substances (PFAS) in the Environment
Oral Presentation

Prepared by E. Walters, M. Ebitson
Biotage, LLC, 10430 Harris Oaks Blvd., Suite C, Charlotte, NC, 28269, United States

Contact Information: [email protected]; 603-401-6266


Extraction protocols and analytical methods utilized for detecting Per- and polyfluoroalkyl substances (PFAS) in aqueous samples have evolved rapidly over the past decade. Since the United States Environmental Protection Agency (EPA) released method 537 in 2009, laboratories across the world have made significant progress in their ability to quantify these contaminants. From 2013 – 2015, EPA 537 was utilized for assessment monitoring as part of the Unregulated Contaminant Monitoring Rule (UCMR) 3 program and was updated to EPA method 537.1, shortly thereafter. In late 2019, EPA method 533 and International Organization for Standardization (ISO) Method 21675 were released, which in addition to EPA method 537.1, enable laboratories to quantify up to 37 PFAS analytes with exceptional precision and accuracy. In anticipation of the upcoming UCMR 5 program, it is beneficial to review these top methods utilized for evaluating PFAS in aqueous samples. This study focuses on comparing the extraction protocols and analytical requirements for each method. Results from these methods will be compared to assess method robustness, analyte detection limits, data accuracy and reproducibility. This evaluation demonstrates the value that can be obtained from comparing protocols and analytical results from current methodology to make informed decisions about the future of PFAS monitoring.