Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) in Drinking Water by HPLC with PDA and FL Detection Using Automated Solid Phase Extraction in Accordance with EPA Method 550.1

Drinking Water (Session 2)
Oral Presentation

Prepared by J. Foss, J. Weisenseel, K. Young
PerkinElmer, PerkinElmer, 710 Bridgeport Ave, Shelton, CT, 06484, United States


Contact Information: [email protected]; 203-447-9324


ABSTRACT

Polycyclic aromatic hydrocarbons (PAHs) are among the most ubiquitous organic pollutants. They are created by the combustion of fossil fuels and are always found as a mixture of individual compounds that differ in behavior, environmental distribution, and their effect on biological systems. Due to their often-low level presence in drinking water (low ppb to ppt levels), extensive sample preparation using a large volume of sample (often 1L) is required for analysis by HPLC. As described in EPA Method 550.1, a 1L sample is passed through a C18 solid phased extraction cartridge and analytes are eluted off and concentrated for analysis. This sample preparation procedure can be very labor intensive, introduce variability, and require experienced personnel to perform. Here we present a simple and automated approach to the sample preparation procedure described in EPA 550.1 using the Promochrom SPE-03 multi-channel SPE system. The HPLC method provides a fast separation of 18 PAHs (EPA 550.1 list + 2) in 10 minutes using both PDA and FL detection. Recoveries and method performance data will be presented.