Analysis of Per-and Polyfluoroalkyl Substances (PFASs) in Aqueous, Solid, Biosolids, and Tissue Samples Using Weak-Anion Exchange SPE and LC-MS/MS Analysis According to EPA Method 1633

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by A. Abdelkaoui
United Chemical Technologies, 2731 Bartram Ave, Bristol, PA, 19007, United States

Contact Information: [email protected]; 215-981-7255


Per-and polyfluoroalkyl substances (PFASs) are a diverse group of synthetic organofluorine compounds that are widely used in industrial applications and consumer products. PFASs are persistent in the environment, are resistant to degradation, and are known to bioaccumulate in humans and wildlife. PFASs have historically been analyzed in drinking water according to EPA 537 (14 compounds) and 537.1 (18 compounds) and EPA 533 which has been validated for the analysis of multiple short-chain PFAS’s, including telomers and precursor compounds, that cannot be measured by EPA 537.1. EPA method 1633 is a currently a draft method for the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous, Solid, Biosolids, and Tissue Samples by LC-MS/MS.
This Poster outlines the analysis of PFAS’s in Aqueous, Solid, Biosolids, and Tissue Samples according to EPA 1633 utilizing UCT’s Enviro-Clean® polymeric weak-anion exchange (WAX) SPE cartridges. LC-MS/MS analysis was carried out using a Selectra® C18 HPLC analytical column, while a short (5cm) C18 delay column was used to reduce potential PFAS contamination from the HPLC system.. For quantitation, a seven-point calibration (0.2-25 ng/mL) was performed, and all compounds were found to be linear with R2 values > 0.99. The extraction method was evaluated by spiking reagent water samples with PFAS’s at 10 and 80 ng/L. Recoveries of all analytes were within a range of 70-130% and RSD values <20%.