Analysis of PFAS Extractables in Millex® Syringe Filters Using LC/MS/MS

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by L. Lozeau, A. Laws, M. Dube
MilliporeSigma, 600 Summit Drive, Burlington, MA, 01803, United States

Contact Information: [email protected]; 17814965656


Guidelines and methods for measuring perfluoroalkyl substances (PFAS) in various matrices have been established by regulatory agencies in recent years, as there is mounting evidence of widespread contamination in the environment and negative impacts to human health. A key consideration for any PFAS method is to avoid contamination from consumables and containers that could come into contact with samples, thereby impacting the accuracy of data. LC/MS/MS-based methods that analyze high-particulate matrices such as wastewater, including SW-846 Method 8327, ASTM D7968, ASTM D7979 and ISO 21675, suggest or require filtration. In such matrices, particulates can considerably reduce LC/MS/MS column lifetime and cause analytical interferences. In this study, LC/MS/MS EPA Method 537.1 for drinking water was used to investigate whether there were PFAS extractables detected in the filtrate from 33mm Millex® syringe filters of 0.22µm and 0.45µm pore sizes. The levels of 28 PFAS compounds, including GenX, were evaluated. Various Millex® syringe filters, including polyethersulfone (PES), nylon and nylon with glass fiber pre-filters were tested. Initial data suggests that PES Millipore Express® syringe filter devices did not demonstrate detectable levels of any of the PFAS compounds tested, at the reporting limit or minimum detection limit of the instrument. Therefore, Millex® syringe filters may be used as a sample preparation technique for analytical methods to characterize PFAS compounds in high-particulate matrices.