Validation Studies of EPA Method 537.1 for Monitoring Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) in Drinking Water Using UHPLC with a Triple Quadrupole Mass Spectrometry

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by M. Cyr, J. Weisenseel, M. Costanzo
PerkinElmer, 710 Bridgeport Ave, Shelton, CT, 06484, United States

Contact Information: [email protected]; 12033796008


Growing environmental and health concerns about Per- and Polyfluoroalkyl Substances (PFAS) have led to stricter and more extensive regulations of these substances in drinking water, ground water, soil and food over the past decade. PFAS are man-made chemicals used in a wide variety of commercial products like nonstick cookware, food packaging, paints, clothing, fire retardants and surfactants since the 1940’s. Due to their inert nature, PFAS are persistent and have been found to accumulate throughout the environment. Originally considered biologically inactive, recent research has revealed their toxicity to humans and wildlife leading to stricter global regulations restricting their levels in food, water, air and soil. EPA Method 537.1 is a widely used method for the determination of selected PFASs in drinking water by solid phase extraction and liquid chromatography/tandem mass spectrometry. This poster will discuss the validation of EPA 537.1 on the QSight 220 LC/MS/MS and the many pitfalls of implementing this method. The QSight 220 demonstrates excellent sensitivity, precision and accuracy running EPA Method 537.1 that provides accurate results for PFAS lower than any current regulatory limits.