Analysis of 6-PPD-Quinone in Environmental Waters.

New Organic Monitoring Techniques
Poster Presentation

Prepared by R. Marfil-Vega, J. Davis, E. Wang, S. Olendorff, C. Gilles
Shimadzu Scientific Instruments, 7102 Riverwood Drive, Columbia, MD, 21046, United States

Contact Information: [email protected]; 410-910-0884


Attention was recently brought to N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6-PPD-quinone) because of its role in the mortality of Coho salmon population in the Pacific Norwest streams. 6-PPD-quinone is an oxidation product of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD). 6-PPD is used ubiquitously in tire rubber as an antioxidant. 6-PPD when released from vehicle tires gets converted to 6-PPD-quinone by reaction with ozone; the chemicals find their way to the streams where Coho salmon inhabits through stormwater run-off. Occurrence of 6-PPD-quinone is linked to a major environmental emerging concern, the ubiquitous distribution of tire-ware particles in the environment. In this work we evaluated the performance of LCMS-8060 triple quadrupole mass spectrometer for the analysis of 6-PPD-quinone. Excellent sensitivity and linearity (r2>0.99) were observed in the 0.01-100 ng/mL range. The overall accuracy for neat standards was in 85-122% range. The spike recovery of 6-PPD-quinone in surface water from two different sources (river and stream) was excellent and ranged between 83-100% at varying spike levels (0.1, 1, 10, and 50 ng/mL) with no sample pre-concentration or clean-up step.