Progress in Microbiological Monitoring Methods

Current Topics in Microbiology
Oral Presentation

Prepared by Y. Chambers-Velarde
General Dynamics Information Technology, 6361 Walker Lane, Suite 300, Alexandria, VA, 22310, United States

Contact Information: [email protected]; 703-485-4265


In the last few years, a number of new microbiological methods have been published and made available to stakeholders for monitoring ambient waters and wastewaters.

Recently, EPA worked with stakeholders to optimize and validate two methods for coliphage for monitoring ambient waters and wastewaters for male-specific and somatic coliphage. EPA leveraged Method 1602 that had been optimized and validated to support the groundwater rule.
In an effort to provide stakeholders with validated methods for microbial source tracking (MST), EPA validated two MST molecular methods that use human-specific markers and made them available on EPA’s CWA methods site in the last year. As technology continues to evolve, EPA continues to work with stakeholders to look at ways to enhance environmental monitoring and address analytical gaps, including qPCR methods that provide results in hours instead of days.

In addition, EPA continues to work with laboratories and commercial vendors through the alternate test procedure program to approve alternate methods for limited and nationwide use under the Clean Water Act.