Application of Capillary Electrophoresis (CE) Coupled with Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) for Simultaneous Speciation Analysis of Arsenic and Selenium in Environmental Samples
Poster Presentation
Prepared by C. Marvin1, B. He2, G. Jiang2, S. Wall1
1 - Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, DE, 19711, United States
2 - Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, State Key Laboratory of Environmental Chemistry and Ecotoxicology, Beijing, 100085, China
Contact Information: [email protected]; 302-636-8423
ABSTRACT
The presence of arsenic, selenium in the environment raises concern about their potentially toxic properties. This work describes the coupling of CE with ICP-MS for the simultaneous separation and determination of arsenic and selenium in environmental matrices. Using a direct-injection high-efficiency nebulizer (DIHEN) interface allowed for the baseline separation an determination of six arsenic species, including arsenite (As(III), arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC), and five selenium species such as sodium selenite (Se(IV)), sodium selenate (Se(VI)), selenocysteine (SeCys), selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) in a single run. Detection limits were ranged between 0.11−0.37 μg/L for the six arsenic compounds and 1.33−2.31 μg/L for the five selenium species. Repeatability (RSD, n = 6) of both migration time and peak area were better than 2.68% for arsenic compounds and 3.28% for selenium compounds. The method has been successfully applied to the determination of arsenic and selenium species in the certified reference materials DORM-3, water, urine, and fish samples.
Poster Presentation
Prepared by C. Marvin1, B. He2, G. Jiang2, S. Wall1
1 - Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, DE, 19711, United States
2 - Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, State Key Laboratory of Environmental Chemistry and Ecotoxicology, Beijing, 100085, China
Contact Information: [email protected]; 302-636-8423
ABSTRACT
The presence of arsenic, selenium in the environment raises concern about their potentially toxic properties. This work describes the coupling of CE with ICP-MS for the simultaneous separation and determination of arsenic and selenium in environmental matrices. Using a direct-injection high-efficiency nebulizer (DIHEN) interface allowed for the baseline separation an determination of six arsenic species, including arsenite (As(III), arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC), and five selenium species such as sodium selenite (Se(IV)), sodium selenate (Se(VI)), selenocysteine (SeCys), selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) in a single run. Detection limits were ranged between 0.11−0.37 μg/L for the six arsenic compounds and 1.33−2.31 μg/L for the five selenium species. Repeatability (RSD, n = 6) of both migration time and peak area were better than 2.68% for arsenic compounds and 3.28% for selenium compounds. The method has been successfully applied to the determination of arsenic and selenium species in the certified reference materials DORM-3, water, urine, and fish samples.
